Product Reference: 060030   

Specifications: 10 Pieces/ box (15 Slices / Piece)

Application: Widely used in drinking water, natural mineral water for drinking coliform detection of source water.

Inspection methods:

1.Inoculation:

According to contamination of samples, not diluted or select the appropriate dilution, each draw five of 10 mL and applied to the five large paper bag; five of 1 mL  applied to five bags of small pieces of paper; then another 1 mL aliquot diluted 10-fold, draw five 1 mL were applied to five bags of small pieces of paper. Then test the paper by hand gently flatten the bag vertically upward to 18 ~ 24h culture incubator (35 ~ 37 ºC) inside, and then observe the results.

2.Results to determine:

Negative results: violet blue paper unchanged, regardless of flush or red spots which is judged as coliform negative.

Positive results: showing red spots or flush on the paper, and its surrounding yellow or yellow throughout the paper which is judged as positive for coliform bacteria.

3.Result report:

 Coliform MPN check the appropriate table based on the number of positive reaction paper reports per 100mL MPN values in the detection of coliform bacteria samples (most probable number).

Note:

1.Should be protected from light hypothermia (10 ºC below) to save, and pay attention to moisture, if pink was found in the paper then the product is unavailable.

2.Confirmatory test methods

To make confirmation test, using sterile forceps aseptically suspicious paper transplanted to lactose fermentation tube, set in 37 ºC culture for 24 hours, its produce acid and gas were judged as positive for coliform bacteria, otherwise judged as negative .

Specifications: 10 Pieces/ box (15 Slices / Piece)

1.Offering  Sample for Testing .

2.R & D team to provide technical support.

3.Provide packaging, labeling OEM customization.

Q1:

Sometimes color of dehydrated culture media  between batches have subtle differences,would this affect test results?

A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.

Q2:

After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?

A2: The reason may be:

The required hydration in the preparation process have not been done completely .That is to say   distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.

Q3:

Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?

A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.

Q4:

During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?

A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.